Statistics For Genomics Batch Effects

Summary

This lecture is part of the course "Introduction to The models behind RMA and fRMA explained. At the end quality assessment comes up and I show some examples of the utility of ... Fundamental idea of differential gene expression testing, and overview of I briefly explain the biology of DNA methylation, CpG Islands and measurement technology (microarrays and sequencing). I describe base calling for next generation sequencing In this video (recorded live in class) I give a brief introduction to next generation sequencing. I describe the technology and some ... I motivate and describe the normal+exponential model used by RMA to background correct microarray intensities. I also describe ... Is a pretty extreme case most of the time you may not see that strong class during effect or On UMAP, your cells cluster by batch — not biology.