Object classification to identify markers with QuPath
1 - QuPath tutorial introduction
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Last Updated: September 27, 2026
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Summary
The previous tutorial is at youtube.com/playlist?list=PL4ta8RxZklWkPB_pwW-ZDVAGPGktAlE5Y This time, we look at ... 00:51 Data set 01:50 Creating a This video shows how to train a pixel classifier to segment epithelium and stroma in H&E images with Matt Cecchini demonstrates how to use QuPath to perform positive cell detection on Ki67 stained slides. The tutorial covers adjusting detection parameters, creating density maps to identify hotspots, and training a classifier to distinguish tumor cells from stroma for more accurate quantification. An objjectclassifier is trained in In this tutorial is about pathology image annotation in Hematoxylin and DAB stain vectors set to Von Kossa pink and black.